UpSetR: better than a venn diagram

Here are some links for you to explore the use of UpSet plots as an alternative to a venn diagram. https://caleydo.org/tools/upset/ https://cran.r-project.org/web/packages/UpSetR/vignettes/basic.usage.html Above the first graph shows 79 genes overlapping between reproductive and sterile maternal bias. In the second graph that overlap is only 73 as some of these genes also overlap with another category … Continue reading UpSetR: better than a venn diagram

Confirmation of Differential or Allele-Specific Methylation

Here is a brief pipeline for confirming allele specific methylation (or differential meth with some modifications) in known genes (like qPCR for gene expression). The above protocol was specifically for confirming methylation in different alleles. However this could be adapted to confirm methylation levels in known genes between different samples. Also the TOPO closing says … Continue reading Confirmation of Differential or Allele-Specific Methylation