Adapted from Abcam Histone Western Blot Protocol, Invitrogen NuPAGE Technical Guide and Dr Ben Cooper’s Western Blotting Protocol for HSP90 v5. Histone Extraction - using the Abcam Histone Extraction Kit Prepare reagents: 1x Pre-Lysis Buffer: dilute 1ml of 10x buffer with 9ml distilled water. Balance DTT Buffer: add 1μl of DTT solution to 500μl Balance … Continue reading Western Blot for Histone Proteins Protocol →