EDIT: no sooner do I publish this than I discover that there is actually a kit that combines Trizol extraction and the PureLink columns.
We’ve got a couple of these Trizol protocols hanging around the lab, just thought it might be a good idea to save them here for posterity. Trizol extraction is good for lysing difficult tissues (such as chitinous insect tissue) and extracting pure RNA for sensitive downstream applications. The protocol as shown below should be fine for RNA-seq, and you can treat it afterwards with DNase if you’re worried about contamination. Alternatively (and this is my preference), you can combine this with column purification such as the PureLink Mini/Micro kit. This saves you having to wash RNA pellets, which can sometimes be barely visible. What I do in this case is follow the protocol up to stage 13, and then switch to the column protocol, joining at stage 6 (page 26) in which you add 70% ethanol to the aqueous phase.
Social Epigenetics Lab 